Sheikh Haydar's son Ismail founded the Safavid Empire by defeating the ruler of Aq Qoyunlu in Tabriz in 1501. Safavid Empire was the longest-lived state which was founded in Iran during the Islamic period. After the foundation, Safavid Empire began to struggle with the Ottoman Empire in the west and Uzbeks in the east. Because, Safavid Empire has found itself in two Sunni world immediately after the establishment. In this struggle, Shah Ismail wanted to remove turmoil by sending his disciples through the territory of the Ottoman Empire in Anatolia. He also knew that the trends of Turkmen tribes of Anatolia comes from the time of his ancestors. Eventually, Safavid Empire was established by the Anatolian Turkish tribes. In so much that the sustaining element of the state has been the Turks. But when the Turkmen tribes began to fight each other to dominate the state, the central authority of the Safavid Empire weakened. Until the Shah Abbas’s reforms in the army and the state, the Qizilbash Turkmen tribes kept their positions in the administration of the Safavid Empire. The most effective Turkmen tribes in this period were Turkmen, Tekelu, Shamlu and Ustacalu tribes. In this article, we will try to explain the activities of these tribes.